Endosymbiotic Theory of Eukaryotic Cell Development

Endosymbiotic Theory of Eukaryotic Cell Development Abstract The defining feature of eukaryotic cells is that they contain membrane-bound organelles and a true nucleus. The endosymbiotic theory is based upon the idea that eukaryotic cells evolved in steps beginning with the stable incorporation of chemo-organotrophic and phototrophic symbionts from the domain bacteria. This essay reviewed the evidence that supports this theory. After investigating the molecular, physiological and morphological evidence, it is almost certain that chloroplasts and mitochondria are from the domain bacteria, and that many of the genes required for the survival of these organelles are contained within the nuclear DNA of the eukaryotic cell rather than the organelles own independent DNA. It is for this reason that I believe that endosymbiosis was the process whereby eukaryotes began to form and evolve. It was found that the genome of a protozoan, Reclinomonas, contained all the protein-coding found in sequenced mitochondrial genomes, providing support for the specul ative process of endosymbiotic gene transfer. The hydrogen hypothesis seems to be the most likely scenario for the formation of eukaryotes, which explains the need for compartmentalisation with increasing host genome size to improve efficiency of function throughout the cell, and the chimeric nature of eukaryotes. Introduction Based upon data collected from slow decaying radioactive isotopes, Earth is thought to have formed approximately 4.55 billion years ago. From this time of origin, a continual process of geological and physical change has occurred, which created conditions leading to the origin of life about 4 billion years ago. Life is thought to have undergone the process of evolution, defined as DNA sequence change and the inheritance of that change, often under the selective pressures of a changing environment. (1) Microfossil evidence suggests that unicellular eukaryotes arose on Earth approximately 2 billion years ago, after the development of an oxic environment and the invention of respiratory metabolism in cyanobacteria. This timing infers that the availability of oxygen was a large influence on the biological evolution that led to the emergence of Eukarya. (1) The defining characteristic of eukaryotes is the presence of a well-defined nucleus within each cell. (2) Typical eukaryotic cells contain a membrane bound nucleus and organelles enclosed by an outer plasma membrane; these organelles are organised into compartmentalised structures which have their own function(s) within the cell, often working together with other organelles to complete vital biological processes. This compartmentation in cells is essential in organisms as it allows differing compositions of nutrients to exist inside each compartment as opposed to outside, creating perfect conditions for biochemical reactions to occur.(3) The differences between eukaryotes and prokaryotes are shown in Table 1: Mitochondria are membrane-bound organelles found in the cytoplasm of most eukaryotic cells and are most concentrated in cells associated with active processes, such as muscle cells which constantly require energy for muscle contraction. The two surrounding membranes that encompass a mitochondrion differ in function and composition, creating distinct compartments within the organelle. The outer membrane is regular in appearance and composed of proteins and lipids, in roughly equal measure, whilst the outer membrane contains porin proteins rendering it more permeable. The inner membrane is only freely permeable to oxygen, water and carbon dioxide; it contains many infoldings, or cristae, that protrude into the central matrix space, significantly increasing the surface area and giving it an irregular shape. As can be seen in Figure 1, mitochondria contain ribosomes and have their own genetic material, mitochondrial DNA (mtDNA), separate from the nuclear DNA. (4) Mitochondria are the principle sites of ATP production- in a process known as oxidative phosphorylation. Products of the Krebs cycle, NADH + H+ and FADH2, are carried forward to the electron transport chain (ETC) and are oxidised to NAD+ and FAD, releasing hydrogen atoms. These hydrogen atoms split to produce protons and electrons, and the electrons are passed down the ETC between electron carriers, losing energy at each level. This energy is utilised by pumping the protons into the intermembranal space causing an electrochemical gradient between the intermembranal space and the mitochondrial matrix. The protons diffuse down the electrochemical gradient through specific channels on the stalked particles of the cristae, where ATPsynthase located at the stalked particles, supplies electrical potential energy to convert ADP and inorganic phosphate to ATP. In mammalian cells, enzymes in the inner mitochondrial membrane and central matrix space carry out the terminal stages of glucose and fatty acid oxidation in the process of ATP synthesis. Mitochondria also play an important role in the regulation of ionised calcium concentration within cells, largely due to their ability to accumulate substantial amounts of calcium. (3)(5) Chloroplasts are membrane-bound organelles found within photosynthetic eukaryotes. Chloroplasts are surrounded by a double membrane, the outer membrane being regular in appearance whilst the inner membrane contains infoldings to form an interconnected system of disc-shaped sacs named thylakoids. These are often arranged in to stacks called grana. Enclosed within the inner membrane of the chloroplast is a fluid-filled region called the stroma, containing water and the enzymes necessary for the light-independent reactions (the Calvin cycle) in photosynthesis. The thylakoid membrane is the site of the light dependent reactions in photosynthesis, and contains photosynthetic pigments (such as chlorophyll and carotenoids) and electron transport chains. Chloroplasts, like mitochondria, contain ribosomes and their own independent DNA (ctDNA), which is central to the theory of endosymbiosis. The structure of a typical chloroplast is shown by Figure 2: Radiant energy is trapped by photosynthetic pigments and used to excite electrons in order to produce ATP by photophosphorylation. The light dependent reactions occur in the thylakoid membrane (Photosystem II or P680) and ultimately, these reactions produce the ATP and NADPH required for photosynthesis to continue in the stroma (where Photosystem I or P700 is located). A series of light independent reactions occur within the stroma producing carbohydrates from carbon dioxide and water using ATP and NADPH. The most supported hypothesis (put forward by Lynn Margulis) for the origin of the eukaryotic cell is that of endosymbiosis which is suitably named as symbiosis occurs when two different species benefit from living and working together. When one organism actually lives inside the other its called endosymbiosis.'(6) The endosymbiosis hypothesis states that the modern, or organelle-containing eukaryotic cell evolved in steps through the stable incorporation of chemo-organotrophic and phototrophic symbionts from the domain Bacteria. In other words, chloroplasts and mitochondria of modern-day eukaryotes arose from the stable incorporation into a second type of cell of a chemoorganotrophic bacterium, which underwent facultative aerobic respiration, and a cyanobacterium, which carried out oxygenic photosynthesis. The beneficial association between the engulfed prokaryote and eukaryote would have given the eukaryote an advantage over neighbouring cells, and the theory is that the prokaryote and eukaryote lost the ability to live independently. (1) Oxygen was an important factor in endosymbiosis and in the rise of the eukaryotic cell through its production in photosynthesis by the ancestor of the chloroplast and its consumption in energy-producing metabolic processes by the ancestor of the mitochondrion. It is worth noting that eukaryotes underwent rapid evolution, most probably due to their ability to exploit sunlight for energy and the greater yields of energy released by aerobic respiration. Support for the endosymbiosis hypothesis can be found in the physiology and metabolism of mitochondria and chloroplasts, as well as the structure and sequence of their genomes.(1) Similarities between modern-day chloroplasts, mitochondria, and prokaryotes relative to eukaryotes are shown in table 2: Molecular Evidence When Margulis proposed the endosymbiotic theory, she predicted that if the organelles really were prokaryotic symbionts, they would contain their own independent DNA. This was proven to be the case in the 1980s for mitochondria and chloroplasts.(7)Furthermore, mitochondrial DNA (mtDNA) was found to have a proportionally higher ratio of guanine-cytosine base pairs than in eukaryotic nuclear DNA, as found in bacteria. These findings are significant as they strongly suggest that mitochondria and chloroplasts are of prokaryotic origin and nature, supporting the possibility that the eukaryotic cell evolved from the stable incorporation of symbionts from the domain Bacteria. Another striking similarity between mitochondria and bacteria is that they both contain 70S ribosomes and contain a comparable order of genes encoding ribosomal proteins a shown in Figure 4: It is only fair that the molecular problems associated with the endosymbiosis hypothesis that have been put forward are considered. Firstly, mitochondria and chloroplasts can only arise from pre-existing mitochondria and chloroplasts, having lost many essential genes needed for survival. It has been suggested that this is because of the large timespan that the mitochondria/chloroplasts have co-existed. During this time, systems and genes that were no longer needed were either simply deleted or transferred into the host genome. Hence, mitochondria and chloroplasts have lost the ability to live independently over time. This supports the endosymbiotic theory as it provides a reason as to why the ancestors of the chloroplasts and mitochondria were able to survive independently whilst chloroplast and mitochondria are unable to do so now. The study of mitochondrial genomes so far has suggested that mitochondrial genomes actually encode less than 70 of the proteins that mitochondria need to function; most being encoded by the nuclear genome and targeted to mitochondria using protein import machinery that is specific to this organelle.(7) It has been found that the genome of Reclinomonas contains all the protein-coding genes found in all the sequenced mitochondrial genomes: (8) The importance of Figure 5 is that it shows that the mitochondrial genome no longer contains many of the protein-coding genes, and hence, mitochondria are no longer able to live independently. The mitochondrial endosymbiont is believed to have belonged to the proteobacteria since several genes and proteins still encoded by the mitochondrial genome branch in molecular trees among homologues from this group. Interestingly, mitochondrial proteins such as the 60- and 70-kDa heat shock proteins (Hsp60, Hsp70), also branch amongst proteobacterial homologues, but the genes are encoded by the host nuclear genome.(9) This can be explained by a theory called endosymbiotic gene transfer which states that during the course of mitochondrial genome reduction, genes were transferred from the endosymbionts genome to the hosts chromosomes, but the encoded proteins were reimported into the organelle where they originally functioned. (7) This theory is central to the endosymbiotic theory, as it explain s the inability of chloroplasts and mitochondria to live independently even though these organelles are believed to have originated from the domain Bacteria. It is also believed that this gene transfer has provided an essential way in which mitochondrial or chloroplast activity can be regulated. The studies of protists raise  the possibility that mitochondria originated at essentially  the same time as the nuclear component of the eukaryotic cell  rather than in a separate, subsequent event. (10) T This would fit in with the hydrogen hypothesis as described later. A further problem to consider is the extent to which genes were transferred to the cell nucleus. Why did some genes remain in the cytoplasmic organelles? This question has been addressed by the Co-location for  Redox  Regulation (CoRR) hypothesis, which states that the location of  genetic information  in  cytoplasmic  organelles  permits regulation of its expression by the reduction-oxidation  (redox) state of its  gene products. Therefore, evolution by natural selection would have favoured mitochondrial or chloroplast cells that had deleted or transferred some genes to the host genome but had kept those that were still beneficial in the regulation of the organelles activity. (11) Physiological Evidence Evidence for the endosymbiosis theory can be found in the physiology of mitochondria and chloroplasts. For example, both mitochondria and chloroplasts have their own protein-synthesising machinery which closely resembles that of Bacteria rather than that of Eukaryotes. Ribosome function in mitochondria and chloroplasts are inhibited by the same antibiotics that inhibit ribosome function in free-living bacteria. Hence, it is no surprise that both these organelles contain 70S ribosomes typical of prokaryotic cells, and show 16S ribosomal RNA gene sequences, a characteristic of certain Bacteria such as Escherichia coli.(1) For example, human mitochondrial ribosomes can be affected by chloramphenicol (an antibiotic used to inhibit protein synthesis), further evidence that mitochondria are likely to be of bacterial origin. Chloramphenicol is a relatively simple molecule containing a nitrobenzene ring responsible for some of the toxicity problems associated with the drug: Chloramphenicol inhibits protein synthesis due to its high affinity for the large (50S) ribosomal subunit, which when bound to chloramphenicol, blocks the action of peptidyl transferase, preventing peptide bond synthesis. It has also been discovered that chloramphenicol prevents the maturation of the 30S ribosomal subunits, decreasing the number of competent subunits and significantly decreasing the proportion of mitochondrial ribonucleoprotein present as monomers. (12) Also, the antibiotic rifampicin which inhibits the RNA polymerase of Bacteria has been found to inhibit the RNA polymerase within bacteria. Proteins of chloroplast or mitochondrion origin, like bacteria, always use N-formylmethionine as their initiating amino acid of their transcript.(13) Mitochondria replicate, like bacteria, only by the process of binary fission inferring that mitochondria did indeed originate from prokaryotes. The completion of the genome sequence of the cyanobacterium Synechocystis, has provided e vidence for the origin of chloroplast translocation apparatus. Just as the endosymbiosis theory predicts, analysis of this sequence showed that three key translocation components within chloroplasts, Toc75, Tic22 and Tic20, evolved from existing proteins within the cyanobacterial genome.(14)Mitochondria and chloroplasts have remarkably similar mechanisms by which ATP is produced. These ATP-generating pathways often include electron transport chains and proton pumps, similar to that found in prokaryotic energy production mechanisms. One of the most recent problems with the endosymbiosis theory is found within the physiology of mitochondria. Mounting evidence suggests that key components of the mitochondrial transcription and replication apparatus are derived from the T-odd lineage of  bacteriophage  rather than from an ÃŽÂ ±-Proteobacterium, as the endosymbiont hypothesis would predict.'(15) It has been discovered that three of the essential elements of the replication and transcription apparatus; the RNA polymerase, the replicative primase-helicase and the DNA polymerase do not resemble those of eubacteria as predicted by the symbiosis theory, but instead appears to resemble proteins encoded by T-odd bacteriophages. However, this does not disprove the theory of endosymbiosis as it is conceivable that numerous mitochondrial genes were acquired together from an ancestor of T-odd phage early in the formation of the eukaryotic cell, at the time when the mitochondrial symbiont was incorporated. (15) Morphological Evidence Another characteristic that further supports the hypothesis is that mitochondria and chloroplasts contain small amounts of DNA that is different from that of the cell nucleus which is arranged in a covalently closed, circular structure, with no associated histones, typical of Bacteria. Mitochondria are surrounded by two membranes, separated by the inter-membranal space and each with a different composition. Mitochondrial membranes more closely resemble membranes found in Gram-negative bacteria in terms of lipid composition than eukaryotic membranes. (16) The inner-membrane infoldings in the mitochondria lends more credibility to the endosymbiosis theory as the cristae are adaptations that increase the surface area of oxidative enzymes, evolutionary analogues to the mesosomal membranes of many prokaryotes (16)Further evidence that mitochondria and chloroplasts are of a prokaryotic origin is the lack of cholesterol in their membranes. This is significant because it is an essential stru ctural component in many eukaryotic membranes, mainly in mammalian cell membrane, but it almost completely absent amongst prokaryotes. Another problem is that recent genetic analysis of small eukaryotes that lack many characteristics that are associated with eukaryotic cells, most importantly mitochondria, show that they all still retain genes involved in the synthesis of mitochondrial proteins. In 1983, the taxon Archezoa was proposed to unite this group of odd eukaryotes, and the belief was that these cells had diverged from other eukaryotes before these characteristics evolved and hence represented primitive eukaryotic lineages. Before the recent genetic breakthrough that shows that these eukaryotes contain genes involved in mitochondrial protein synthesis, molecular work supported their primitive status, as they consistently fell deep into the branches of eukaryotic trees. This recent genetic analysis implies that all these eukaryotes once had mitochondria, suggesting that they evolved after the mitochondrial symbiosis. There is also the question of how the eukaryotic cell arose, including the nature and propert ies of the cell that acquired mitochondria and later chloroplasts, and how the nuclear membrane was formed which touches upon the compatmentalisation within cells and its importance in the functioning of the eukaryotic cell. (7) Formation of the eukaryotic cell There have been two hypotheses put forward to explain how the eukaryotic cell arose. One states that eukaryotes started as a nucleus-bearing lineage that later acquired the bacterial ancestor of the mitochondrion and the cyanobacterial ancestor of the chloroplast by the process of endosymbiosis. This nucleated line then diverged into the lineages giving rise to animals and plants. It is thought that the nucleus arose spontaneously in an early cell. One possible cause for the spontaneous formation of the nucleus is that it arose in response to the increasing genome size of early eukaryotes. (1) The second hypothesis, also known as the hydrogen hypothesis, states that the bacterial ancestor of the mitochondrion was taken up by a member of the Archaea via endosymbiosis, and from this association, the nucleus later emerged, followed by a later acquisition of the cyanobacterial ancestor of the chloroplast. The main difference between these two hypotheses is the position of the mitochondrion relative to the formation of the nucleus in time and hence on the universal phylogenetic tree. The hydrogen hypothesis put forward by William F. Martin and Miklos Muller in 1998, proposes that the eukaryotic cell arose from a symbiotic association of an anaerobic, hydrogen dependent, autotrophic archaebacterium (the host) with a hydrogen producing, oxygen consuming eubacterium (the symbiont), which released molecular hydrogen as a waste product of anaerobic heterotrophic metabolism. (17) The dependence of the host upon the molecular hydrogen as an energy source, produced as a waste product b y the symbiont is thought to be what lead to the association. In this scenario, the nucleus arose following the formation of this stable association between these two kinds of cells, and genes involved in lipid synthesis were transferred from the symbiont to the host chromosome. This may have lead to the synthesis of bacterial (symbiont) lipids by the host, eventually leading to the creation of an internal membrane system, the endoplasmic reticulum and the early stages of a eukaryotic nucleus. As the size of the host genome increased with time, changes were made to maximise the efficiency of replication and gene expression via the process of evolution. Hence, over time, this kind of cell compartmentalised and sequestered the genetic coding information within a protected membrane away from the cytoplasm. The formation of a mitochondrion-containing nucleated cell line was complete, which then later acquired chloroplasts by endosymbiosis. The hydrogen hypothesis has explains the observ ation that eukaryotes are of chimeric nature, containing attributes of both Bacteria and Archaea. (1) Conclusions In summary, molecular, physiological and morphological evidence can be found to support the endosymbiosis theory put forward by Lynn Margulis. Most compelling of which is the numerous similarities between organelles such as chloroplasts and mitochondria with prokaryotes, coupled with the inability of the organelles to live independently despite having their own independent DNA due to most of the genes required for the survival of the organelle being stored in the nuclear DNA of the host. The importance of this should not be underestimated, as it does all but prove that the ancestors of mitochondria and chloroplasts were of a prokaryotic origin and thus were once able to live independently. Therefore, this does lend credibility to the endosymbiosis theory as the symbionts that were allegedly incorporated were likely to have been from the domain bacteria, and that something must have occurred which stopped the symbionts being able to live independently, an event which many scientists n ow believe to be the process of endosymbiotic gene transfer. The hydrogen hypothesis appears to be the likely scenario for how the eukaryotic cell evolved, as it explains the formation of the nucleus as being a response to the growing size of the nuclear genome of the host, which would have maximised efficiency of gene expression. Endosymbiosis also explains why the eukaryotic cell appears to be of a chimeric nature; containing attributes of Archaea (e.g. similar transcription and translation apparatus) and Bacteria (e.g. contain same type of lipids).

Term Essays -- essays research papers

Dreams   Ã‚  Ã‚  Ã‚  Ã‚   Every ninety minutes of every night we’re asleep, a dream unfolds. The short dreams at the beginning of the night are usually no more exciting that the idle thoughts that randomly pop into our heads when we’re awake. But as the night progresses, the length of each dream increases (the last dream can be up to twenty minutes long), the body responds more intensely (increased heartbeat, respiration, muscle twitching), and the dreams become deeper.   Ã‚  Ã‚  Ã‚  Ã‚  Dreams are sometimes said to be ordinary sequences of images that are experienced by the mind during sleep. For thousands of years they were regarded as divine visitations or predictions. Dreams were not really scientifically studied until the 19th century. Today they are still being studied to learn more about man’s nature. Dreaming is a natural ability- we all dream every night. Every one dreams when sleeping at least three to four times every night. The problem however is remembering the dream. But there is a way to remembering dreams. It is believed that dreams are remembered more accurately immediately after awakening during the night rather than in the morning. Remembering dreams takes time, effort, and patience. Researchers discovered that people who tend to forget their dreams show a greater amount of rapid eye movement, which is associated with greater amounts of activity in the dream. However, when these people did remember the content of their dreams, it wa...

Operation Management Case Studies Essay

Operation Management Case Studies Intoduction Case study on Salmon processing facility Question 1 E= $ 6,333.33 Therefore, the additional expenditure for the productivity of the two systems to equal will be $ 6, 333.33 Question 4                   If the cost of energy increases in future, the productivity of facility will be affected negatively and it will decrease. If the cost of the energy increases beyond the estimated additional expenditure of $ 6,333.33, the productivity of the new facility will be much lower than that of the old system and therefore, it will not be advantage to the firm. This will also affect reduce the profitability of the company. Case 2 on Hard Rock Cafe Question 1                   The Hard Rock strategic changes that it has experienced include globalization and changes in its brand through unique services that include rock music. The music has led to the growth of this company. It has managed to open 157 cafe facilities in more than 57 countries. The cafe has also diversified brands to fish and lobsters in Britain. Moreover, it has started shifting its target market from tourists to non tourists’ cities in United Kingdom in addition to leasing cafes in tourism cites such as Nottingham (Heizer, & Render, 2013). Question 2                   Hard Rock has various PRODUCTS that vary from Hamburger, beef, and other tourism attracting meal. The cafe is has also diversified its products to fish and lobsters thus widening its market and meeting the needs of the customer.                   Hard rock cafe moved its LOCATION from domestic market to global market. In addition, it has started opening in new non tourisms LOCATIONS such as Manchester united to prevent risks associated with economic fluctuations that arise from tourism business poor performance.                   Hard rock HUMAN RESOURCE has participated in developing new strategies that has helped to improve the performance the business. For example, the introduction of sound and visuals in dinner events that attract more customers and recruitment of highly trained employees that provides quality services.                   The Hard Rock has improved its  SERVICE by adding unique services that cannot be found in any other place in the world such as music on both quality visual and sounds. They serve food with enthusiasm on their customers (Heizer, & Render, 2013). Question 3 -123825158750Introduction Growth Maturity Decline Standardization Less rapid product changes – more minor changes Optimum capacity Increasing stability of process Long production runs Product improvement and cost cutting Little product differentiation Cost minimization Overcapacity in the industry Prune line to eliminate items not returning good margin Reduce capacity Forecasting critical Product and process reliability Competitive product improvements and options Increase capacity Shift toward product focused Enhance distribution Product design and development critical Frequent product and process design changes Short production runs High production costs Limited models Attention to quality Best period to increase market share R&D product engineering critical Practical to change price or quality image Strengthen niche Cost control critical Poor time to change image, price, or quality Competitive costs become critical Defend market position OM Strategy/Issues Company Strategy/Issues HDTV CD-ROM Color copiers Drive-thru restaurants Fax machines Station wagons Sales 3 1/2† Floppy disks Internet 00Introduction Growth Maturity Decline Standardization Less rapid product changes – more minor changes Optimum capacity Increasing stability of process Long production runs Product improvement and cost cutting Little product differentiation Cost minimization Overcapacity in the industry Prune line to eliminate items not returning good margin Reduce capacity Forecasting critical Product and process reliability Competitive product improvements and options Increase capacity Shift toward product focused Enhance distribution Product design and development critical Frequent product and process design changes Short production runs High production costs Limited models Attention to quality Best period to increase market share R&D product engineering critical Practical to change price or quality image Strengthen niche Cost control critical Poor time to change image, price, or quality Competitive costs become critical Defend market position OM Strategy/Issues Company Strategy/Issues HDTV CD-ROM Color copiers Drive-thru restaurants Fax machines Station wagons Sales 3 1/2† Floppy disks Internet                   The company is still in the growth stage but approaching maturity. This is from the fact that Hard Rock is trying to change its products from beef and Hamburger to lobster and fish, it can be noted that it is trying to improve its products in order to increase its competitiveness. The company is still forecasting on its future market where it want to change from tourists-dependent market to non-tourism dependent market (Heizer, & Render, 2013). The cafe has products that are reliable with little improvement. Reference Heizer, J., & Render, B. (2013). Principles of operation ,management: Sustainability and supply chain management. Pearson Source document

The Complexities of Prosecuting Cyber Crime Essay - 650 Words

Cyber crime and traditional crime have similar identifiable traits when criminal activity is the outcome. The goal of any criminal is to cause harm, threat steal, extortion, to name a few with intent to harm the public good. Local and state law prosecutes traditional crimes through the state attorney. The prosecutor represents the people(entity) of the state. The defendant or the entity charged must prove to the state, by reasonable doubt, their innocent’s of the charges. Individuals that are charged with a crime have the option of hearing their case in front of a judge or a jury. The prosecution can also offer the defendant a plea bargain deal. These deals usually lessen the punishment while also rendering a guilty verdict†¦show more content†¦Microsoft had brought charges against two members of the Zeus Botnet crime ring in 2012. However, two individuals were already in jail serving time for spreading Zeus malware in the United Kingdom. As part of the Microsoft Active Response for Security (MARS) program, the company filed a lawsuit against a group of unknown defendants-identified as John Does in the original complaint-for violation of a number of civil statutes.The two defendants were identified as, Yevhen Kulibaba and Yuriy Konovalenko, and amended the complaint (Lemos, 2012 ). Since a case was already brought against the two suspects and the crime organization. It was a going to be a challenge for Microsoft to file charges for basically the same crime. What Microsoft was able to accomplish according to the Microsoft Senior Attorney for Microsofts Digital Crimes Unit, Richard Boscovich was the reduction of Zeus botnet occurrences. Microsoft was able to shutdown 800 domain accounts and to servers located in the united states from spreading the botnet. Last year, federal prosecutors charged a cyber crime ring with stealing prepaid debit cards data. And using that data to draw unlimited amounts of money from ATM’s ,Loretta Lynch, the U.S. Attorney in Brooklyn, said in a statement. This was a 21st century bank heist that reached across the Internet and stretched around the globe. In the place of guns and masks, this cybercrime organization used laptops and theShow MoreRelatedEssay on The Prosecution and Tragic Death of Aaron Swartz1137 Words   |  5 Pagesfollowing the rejection of a possible plea agreement by Ortiz, Swartz committed suicide by hanging. Immediately a public out lash immerged against MIT and the Federal Prosecution. The Hacktivist group Anonymous, subsequently conducted a series of cyber-attacks against MIT and the Department of Justice for its involvement. I have provided a timeline of the claimed attacks by Anonymous associate with the Aaron Swartz case. Please note that the tribute was posted on MIT’s external website the day followingRead MoreAccounting Information System Chapter 1137115 Words   |  549 Pagesreport to communicate to all users, the problems the corporation faces include the following.  · In an attempt to reach several audiences, a company may include information for each audience. Consequently, the annual report may grow in size and complexity to the point where it contains more information than many users want to receive or are able to comprehend, i.e., information overload. In some cases, technical concepts may be reduced to concepts that are more common; this reduces precision and

Civic culture In Yugoslavia Essay - 1647 Words

One definition of ethnicity reads as followed â€Å"identity with or membership in a particular racial, national, or cultural group and observance of that group’s customs, beliefs, or language†. Ethnicity defines many of us but a few want our definition to be â€Å"cleansed† and this is when civil conflict arises. Ethnicity is frequently cited as an explanation for conflicts in the post cold war era. Yugoslavia, a prime example of a system that encountered ethnic conflict that led to a violent civil war, consisted of six republics and two autonomous districts. The government at the time wanted control of these republics but the republics wanted to be free from government control. There was a push by several ethnic groups to form their own sovereign†¦show more content†¦These congruities of blood, speech, custom, and so on, are seen to have an ineffable, and at times overpowering, coerciveness in and of themselves. 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Therefore, this study focuses on the relationship among gender, age, ethnicity, and education background on employee’s performance in NATIONAL BANK. 1Read MoreAlternative Dispute Resolution Mechanism21967 Words   |  88 Pagesnegotiation and mediation, with examples of their application in national and international water conflicts. Conflict is endemic to human society, among individuals and groups, and it is important to manage it. We find stories in the Bible, in the Islamic culture, among Native Americans, First Nations in Canada, and many other traditions that describe processes that have been used from the earliest times to find peaceful solutions to various disputes, and much can be learned from the past. In recent decades

A Sociological View of Rastafarianism Essay - 3688 Words

Organized religion is a duality between the religion and the church which represents it. Sometimes the representation of the religion is marred and flawed to those who view it because of the bureaucracy contained within. Unknown to those who gaze upon the dissolved morals and values of what is perceived to be the contradiction known as modern religion, it was never intended to be this way. Most religions started off as a sect, a minor detail on the fringes of the society it never wanted to represent. Rastfarianism is such a sect. The differences between Rastafarianism and a normal â€Å"mainstream† religion are numberless, including: no set membership, no authoritative leader, no offices of authority, no trained clergy and no involvement with†¦show more content†¦He saw African civilization as anterior to all others and used bible verses which were easily interpretable to portray Africans as the chosen people mentioned in the bible, as in Psalm 68: â€Å"Princes shal l come out if Egypt and Ethiopia shall stretch forth his hands onto God† (Barret 78). Garvey’s persistence culminated in the crowning of Ras Tafari as Negus of Ethiopia. He took the name Haile Selassie and added â€Å"King of Kings† and the â€Å"Lion in the Tribe of Judah†, placing himself in the legendary line of King Soloman, and therefore, in the same line as Jesus Christ of Roman Catholicism. Out of this came Rastafarianism which took over Jamaica at a time when it was â€Å"in a low tide economically and socially. Socially, people experienced the brunt of the Depression as well as disaster due to a devastating hurricane. Politically, colonialism gripped the country and the future of the masses looked hopeless. Any doctrine which that promised a better hope and a better day was ripe for hearing† (Barret 84). Weber analyzed conditions such as these as a theodicy of suffering. â€Å"One can explain suffering and injustice by refrying to individual sin committed in former life, to the guilt of ancestors . . . to the wickedness of all people. As compensatory promised one can refer to hopes of the individual for a better life in the future of this world or to the for the successors, or to a better life in the hereafter† (Weber 275). In other words, those who are disadvantaged in aShow MoreRelatedA Sociological View of Rastafarianism3718 Words   |  15 Pagesthe religion is marred and flawed to those who view it because of the bureaucracy contained within. Unknown to those who gaze upon the dissolved morals and values of what is perceived to be the contradiction known as modern religion, it was never intended to be this way. Most religions started off as a sect, a minor detail on the fringes of the society it never wanted to represent. Rastfarianism is such a sect. The difference s between Rastafarianism and a normal mainstream religion are numberlessRead More Nature in the Rastafarian Consciousness Essay example3436 Words   |  14 PagesNature in the Rastafarian Consciousness Living in harmony with the environment and the laws of Nature is one of the central ideas of Rastafarianism. To live in accordance with the Earth is to live in accordance with Jah; it is incorporated into the morality that is Rastafarian consciousness. The Rastas reverence for nature is influenced by the traditional African religions which are still practiced in Jamaica and which have also influenced Christianity on the island tremendously. Hinduism, tooRead MoreEssay Webers Approach to Religion and Sociology1706 Words   |  7 Pages Webers general approach to sociology is known as verstehen sociology; that human action is directed by meaning and that action can only be understood by appreciating the world-view of the social actor concerned. Since religion is an important component of the social actors world-view, religious beliefs can direct social action, and hence bring about social change. In The Protestant Ethic and the Spirit of Capitalism, Weber explores the relationship between religiousRead MoreRastafarian79520 Words   |  319 Pagesus to survive and thrive This page intentionally left blank Foreword One of the most useful things about Ennis Edmondss Rastafari: From Outcasts to Culture Bearers is that it correctly traces the connection between the emergence of Rastafarianism and the history of resistance and black consciousness that has been part of the Jamaican experience for years. The truth is that there has always been a committed Jamaican counter- culture that celebrates and sees redemption in Africa and rejectsRead MoreSlavery in Latin America vs Us3432 Words   |  14 Pagesabout many changes, with respect to African-Americans and black culture. Those changes had long lasting effects, not only on how blacks view and are viewed in society, but also on how the destruction of our culture influenced our current life-style today in United States and Latin America. Skin color is still an important factor in today’s society, due to the sociological affects from slavery, which started over 500 years ago. After the conquest, Latin America was referred to as the New World,

Brand Prestige and Functioning-Free-Samples-Myassignmenthelp.com

Question: Analyse similarities between two different watches. Answer: Brand Prestige and functioning The branding that is being undertaken by Richard Mille is based on the functionality of the watch based on the utilization of the high tech materials and the most appropriate design to fit in the requirements of the customers who are after the prestige related aspects of the brands. The elegant and classic looks of the watch has attracted the attention of the fashion conscious customers. The watch is designed in Sweden with a superior classic look, which helps in determining the brand. The proper functioning of the organization is based on the understanding of the needs of the customers to fulfill their criteria of adding the value of prestige which is determined by the product that is being used by the same (Hanzaee and Taghipourian 2012). Therefore, the branding of the product s is again based on the choices of the customers where the customers identify the brand as a sign of the elegance. On the other hand, the branding also ensures the customers of the proper services and the fun ctioning of the commodity that is being chosen by the customers. The branding of prestige oriented products and the branding of function-oriented products in todays world is based on the choice of the customer and the manner in which the customers perceives the product available in the market. It is kind of transferred epithet that affects the choice of the product made by the customers, which is determined by the branding that is used by the organization in the market (Esmaeilpour 2015). On the other hand, the branding of the Daniel Wellington Watch is based on the factors of the functionality that is a major concern for the customers. The functionality of the watch has helped in attracting the attention of the customers in the market (Theng So, Grant Parsons and Yap 2013). The functioning of the watch has helped in determining the different aspects of the customer requirements. The branding of the watch is based on creating awareness among the customers. It has helped in understanding the different aspects of the change that is required to be undertaken by the manufacturers in order to adhere to the needs of the customers in the market (Viktoria Rampl and Kenning 2014). On the other hand, the different perspectives of the customers adds to the loyalty of the same in the market structure (Vigneron and Johnson 2017). The customer loyalty pays a good price for the organization as it helps in bringing about improvements in the product that is manufactured by the organiz ation. The company produces its products, which is based on the requirements of the customers based on the functioning of the product (Keller 2017). The similarity of the branding of the two most elegant watch manufacturers is based on the assurance of the quality. Watch is a passion for the admirers therefore, branding of the watches play an important role in satisfying the emotional needs of the customer. On the other hand, the elegance of the Richard Mille watch and the sporty functionality of the Daniel Wellington Watch is based on attracting the target customers. Branding thereby plays a major role in serving the quality products and services to the requirements of the customers. The similarities in the branding of the watches help in identifying the various demands of the customer and thereby reaching out to the target groups. References Esmaeilpour, F., 2015. The role of functional and symbolic brand associations on brand loyalty: A study on luxury brands.Journal of Fashion Marketing and Management,19(4), pp.467-484. Hanzaee, K.H. and Taghipourian, M.J., 2012. The effects of brand credibility and prestige on consumers purchase intention in low and high product involvement.Journal of Basic and Applied Scientific Research,2(2), pp.1281-1291. Keller, K.L., 2017. Managing the growth tradeoff: Challenges and opportunities in luxury branding. InAdvances in Luxury Brand Management(pp. 179-198). Palgrave Macmillan, Cham. Theng So, J., Grant Parsons, A. and Yap, S.F., 2013. Corporate branding, emotional attachment and brand loyalty: the case of luxury fashion branding.Journal of Fashion Marketing and Management: An International Journal,17(4), pp.403-423. Vigneron, F. and Johnson, L.W., 2017. Measuring perceptions of brand luxury. InAdvances in Luxury Brand Management(pp. 199-234). Palgrave Macmillan, Cham. Viktoria Rampl, L. and Kenning, P., 2014. Employer brand trust and affect: linking brand personality to employer brand attractiveness.European Journal of Marketing,48(1/2), pp.218-236.